An Introduction to Molecular Biotechnology

FUNDAMENTALS OF CELLULAR AND MOLECULAR BIOLOGY The Cell as the Basic Unit of Life Structure and Function of Cellular Macromolecules Structure and Functions of a Cell Biosynthesis and Function of Macromolecules (DNA, RNA, and Proteins) Distributing Proteins in the Cell (Protein Sorting) Evolution and Diversity of Organisms STANDARD METHODS IN MOLECULAR BIOTECHNOLOGY Isolation and Purification of Proteins Peptide and Protein Analysis with Electrospray Tandem Mass Spectrometry Isolation of DNA and RNA Chromatography and Electrophoresis of Nucleic Acids Hybridization of Nucleic Acids Use of Enzymes in the Modification of Nucleic Acids Polymerase Chain Reaction DNA Sequencing Genome Editing Cloning Procedures Expression of Recombinant Proteins Omics technologies Patch Clamp Method Cell Cycle Analysis Microscopic Techniques Laser Applications KEY TOPICS Genomics and Functional Genomics Bioinformatics Cellular Systems Biology Protein-Protein and Protein-DNA Interaction Drug Research Drug Targeting and Prodrugs Molecular Diagnostics and Personalized Medicine Recombinant Antibodies and Phage Display Transgenic and Gene-Targeted Mice Plant Biotechnology Industrial Biocatalysis BIOTECHNOLOGY IN INDUSTRY The Biotech Industry, Markets, and Opportunities Patents in the Molecular Biotechnology Industry: Legal and Ethical Issues Drug Approval in the European Union and United States Founding a Biotech Company APPENDIX Further Reading Abbreviations Subject Index

Michael Wink studied biology and chemistry in Bonn and was awarded his doctorate from TU Braunschweig in 1980. After gaining his lecturing qualification in 1984/1985, he was awarded a Heisenberg grant by the German Research Council to work at the Max Planck Institute for Breeding Research in Cologne and from then at the Gene Center of Ludwig-Maximilians University in Munich. Following a chair for Pharmaceutical Biology at Mainz University in 1988, he accepted the post of Professor for Pharmaceutical Biology at the University of Heidelberg one year later. His areas of interest include pharmaceutical research, molecular biotechnology, and medicinal plants, as well as research into natural products and evolution.