Avian and Reptilian Developmental Biology

Preface... Table of Contents... Contributing Authors... 

Commentary 1  Some Thoughts on Experimental Design Claudio D. Stern 

Part I Genomics and Transcriptomics 2  Comparative Genomics as a Foundation for Evo-Devo Studies in Birds Phil Grayson, Simon Y.W. Sin, Timothy B. Sackton, and Scott V. Edwards 3  A Step-by-Step Guide to Assemble a Reptilian Genome Asier Ullate-Agote, Yingguang Frank Chan, Michel C. Milinkovitch, and Athanasia C. Tzika 4  Genomic and Transcriptomic Analyses of Avian Sex Chromosomes and Sex-Linked Genes Jilin Zhang, Jing Li, and Qi Zhou 5  Systems Biology Analyses in Chicken: Workflow for Transcriptome and ChIP-Seq Analyses using the Chicken Skin Paradigm Yung-Chih Lai, Randall B. Widelitz and, and Cheng-Ming Chuong 6  Application of a CAGE Method to an Avian Development Study Ruslan Deviatiiarov, Marina Lizio, and Oleg Gusev

Part II Genetic Manipulation 7  CRISPR/Cas9 in the Chicken Embryo Valérie Morin, Nadège Véron, and Christophe Marcelle  8  Fluorescent Quail: A Transgenic Model System for the Dynamic Study of Avian Development David Huss and Rusty Lansford 9 Lentiviral-Mediated Transgenesis in Songbirds Wan-chun Liu, Marian Hruska-Plochan, and Atsushi Miyanohara 10 In Ovo Electroporation Methods in Chick Embryos Hidekiyo Harada, Minoru Omi, and Harukazu Nakamura 11 Genetic Manipulation of the Avian Urogenital System using In Ovo Electroporation Claire E. Hirst, Olivier Serralbo, Katie L. Ayers, Kelly N. Roeszler and Craig A. Smith 12  Enhancer Analyses using Chicken Embryo Electroporation Masanori Uchikawa, Naoko Nishimura, Makiko Iwafuchi-Doi, and Hisato Kondoh 13 Transgene Introduction into the Chick Limb Bud by Electroporation Shogo Ueda, Takayuki Suzuki, and Mikiko Tanaka 

Part III Stem Cells 14 Chicken Induced Pluripotent Stem Cells: Establishment and Characterization Aurelie Fuet and Bertrand Pain 15 Isolation and