Baculovirus and Insect Cell Expression
(Sprache: Englisch)
The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to...
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The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.
Inhaltsverzeichnis zu „Baculovirus and Insect Cell Expression “
PART I. Introduction1 Useful Tips, Widely Used Techniques And Quantifying Cell Metabolic Behavior
David W. Murhammer
PART II. Baculovirus Molecular Biology/Development of Recombinant Baculoviruses
2 Introduction to Baculovirus Molecular Biology
Barbara J. Kelly, Linda A. King And Robert D. Possee
3 Baculovirus Transfer Vectors
Robert D. Possee And Linda A. King
4 Recombinant Baculovirus Isolation
Linda A. King, Richard Hitchman And Robert D. Possee
5 Gene Expression In Mammalian Cells Using Bacmam, A Modified Baculovirus System
James A. Fornwald, Quinn Lu, Frederick M. Boyce And Robert S. Ames
PART III. INSECT CELL CULTURE
6 Available Lepidopteran Insect Cell Lines
Dwight E. Lynn and Robert E. Harrison
7 Lepidopteran Insect Cell Line Isolation from Insect Tissue
Dwight E. Lynn
8 Development Of Serum-Free Media For Lepidopteran Insect Cell Lines
Leslie C. L. Chan and Steven Reid
9 Routine Maintenance And Storage Of Lepidopteran Insect Cell Lines And Baculoviruses
Dwight E. Lynn and Robert L. Harrison
PART IV. Protein Production with Recombinant Baculoviruses
10 Small-Scale Production Of Recombinant Proteins Using The Baculovirus Expression Vector System
Jian-Ping Yang
11 Recombinant Protein Production In Large Scale Agitated Bioreactors Using The Baculovirus Expression Vector System
Christine M. Thompson, Johnny Montes, Marc G. Aucoin and Amine A. Kamen
12 Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors
Sue H. Kadwell and Laurie K. Overton
13 Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae
Elena Kovaleva and David C. Davis
14 Production Of Virus-Like Particles For Vaccination
Christine M. Thompson, Marc G. Aucoin and Amine A. Kamen
15 Alternative Strategies for Expressing Multi-Component Protein Complexes in Insect Cells
Stephanie Chen
PART V. Recombinant Protein Production with Transformed Insect Cells
16 Transforming Lepidopteran Insect Cells for Continuous
... mehr
Recombinant Protein Expression
Robert L. Harrison and Donald L. Jarvis
17 Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression
Marija Backovic and Thomas Krey
18 Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression
Robert L. Harrison and Donald L. Jarvis
PART VI Baculovirus Development and Production for Use as Insecticides
19Introduction To The Use Of Baculoviruses As Biological Insecticides
Holly J. R. Popham, Tyasning Nusawardani And Bryony C. Bonning
20 Baculovirus Insecticide Production In Insect Larvae
Nikolai Van Beek and David C. Davis
21Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses
Holly J. R. Popham, Mark R. Ellersieck, Huarong Li and Bryony C. Bonning
PART VII. Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System
22 Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants
Hsuan-Chen Wu, Hyung Joon Cha and William E. Bentley
23 Tubular Bioreactor for Probing Baculovirus Infection and Protein Production
Hsuan-Chen Wu, Yu-Chen Hu and William E. Bentley
24Gene Silencing In Insect Cells Using Rnai
Hsuan-Chen Wu, John C. March and William E. Bentley
25 Using the Baculovirus/Insect Cell System to Study Apoptosis
Nor Chejanovsky
26 Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells
Christian Hofmann
Robert L. Harrison and Donald L. Jarvis
17 Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression
Marija Backovic and Thomas Krey
18 Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression
Robert L. Harrison and Donald L. Jarvis
PART VI Baculovirus Development and Production for Use as Insecticides
19Introduction To The Use Of Baculoviruses As Biological Insecticides
Holly J. R. Popham, Tyasning Nusawardani And Bryony C. Bonning
20 Baculovirus Insecticide Production In Insect Larvae
Nikolai Van Beek and David C. Davis
21Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses
Holly J. R. Popham, Mark R. Ellersieck, Huarong Li and Bryony C. Bonning
PART VII. Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System
22 Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants
Hsuan-Chen Wu, Hyung Joon Cha and William E. Bentley
23 Tubular Bioreactor for Probing Baculovirus Infection and Protein Production
Hsuan-Chen Wu, Yu-Chen Hu and William E. Bentley
24Gene Silencing In Insect Cells Using Rnai
Hsuan-Chen Wu, John C. March and William E. Bentley
25 Using the Baculovirus/Insect Cell System to Study Apoptosis
Nor Chejanovsky
26 Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells
Christian Hofmann
... weniger
Bibliographische Angaben
- 2016, 3. Aufl., XII, 508 Seiten, 15 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: David W. Murhammer
- Verlag: Springer, Berlin
- ISBN-10: 1493930427
- ISBN-13: 9781493930425
- Erscheinungsdatum: 29.01.2016
Sprache:
Englisch
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