Cancer Immunotherapy

Isolation of live immune cells from the tumour microenvironment by FACS.- In vitro evaluation of cancer cell immunogenicity and antigen-specific T-cell cytotoxicity by flow cytometry.- Retroviral transduction of human primary T cells followed by real-time T-cell mediated cancer cell cytolysis analysis.- Expansion and retroviral transduction of primary murine T cells for CAR T cell therapy.- In Situ Decellularisation of Tissues applied to the topographical analysis of tumour associated extracellular matrix.- Monitoring cell cytoskeleton variations upon piezoelectric stimulation: Implications for the immune system.- Preparation method and in vitro characterization of nanoparticles sensitive to tumor microenvironment.- A new microfluidic device to facilitate functional precision medicine assays.- Kinetic detection of apoptosis events via caspase 3/7 activation in a tumor-immune microenvironment on a chip.- Hypoxic 3D Tumor Model for Evaluating of CAR-T Cell Therapy In Vitro.- Rapid screening of CAR T cell functional improvement strategies by highly-multiplexed single-cell secretomics.- Genome editing in CAR-T cells using CRISPR/Cas9 technology.- Genetic modification of tumor-infiltrating lymphocytes, peripheral T cells, and T cell model cell lines.- Transposon-based manufacturing of human CAR-T cells.- Redirecting human conventional and regulatory T cells using chimeric antigen receptors.- How to Test Human CAR T cells in Solid Tumors, The Next Frontier of CAR T Cell Therapy.- Nano-Optogenetic CAR-T Cell Immunotherapy.- A non-viral piggyBac transposon-mediated method to generate large-scale CAR-NK cells from human peripheral blood primary NK cells.- Engineering probiotic E.coli Nissle 1917 for release of therapeutic nanobodies.