Computer Optimized Microscopy

Main Steps in Image Processing and Quantification: The Analysis Workflow.- Open Source Tools for Biological Image Analysis.- Proximity Ligation Assay Image Analysis Protocol: Addressing Receptor-Receptor Interactions.- Introduction to ImageJ Macro Language in a Particle Counting Analysis: Automation Matters.- Automated Macro Approach to Quantify Synapse Density in 2D Confocal Images from Fixed Immunolabeled Neural Tissue Sections.- Automated Quantitative Analysis of Mitochondrial Morphology.- Structure and Fluorescence Intensity Measurements in Biofilms.- 3D+Time Imaging and Image Reconstruction of Pectoral Fin during Zebrafish Embryogenesis.- Automated Macro Approach to Remove Vitelline Membrane Autofluorescence in Drosophila Embryo 4D Movies.- Which Elements to Build Co-Localization Workflows? From Metrology to Analysis.- A Triple Colocalization Approach to Assess Traffic Patterns and their Modulation.- Photobleaching and Sensitized Emission Based Methods for the Detection of Förster Resonance Energy Transfer.- In Vivo Quantification of Intramolecular FRET using RacFRET Biosensors Manel.- Cell Proliferation High-Content Screening on Adherent Cell Cultures.- HCS Methodology for Helping in Lab Scale Image-Based Assays.- Filopodia Quantification using FiloQuant.- Coincidence Analysis of Molecular Dynamics by Raster Image Correlation.- 3D Tracking of Migrating Cells from Live Microscopy Time-Lapses.- A Cell Segmentation/Tracking Tool Based on Machine Learning.- 2D+Time Object Tracking using Fiji and ilastik.- Machine Learning: Advanced Image Segmentation using ilastik.