Directed Molecular Evolution of Proteins

IntroductionEvolutionary Biotechnology - From Ideas and Concepts to Experiments and Computer SimulationsUsing Evolutionary Strategies to Investigate the Structure and Function of Chorismate MutasesConstruction of Environmental Libraries for Functional Screening of Enzyme ActivityInvestigation of Phage Display for the Directed Evolution of EnzymesDirected Evolution of Binding Proteins by Cell Surface Display: Analysis of the Screening ProcessYeast n-Hybrid Systems for Molecular EvolutionAdvanced Screening Strategies for Biocatalyst DiscoveryEngineering Protein EvolutionExploring the Diversity of Heme Enzymes through Directed EvolutionDirected Evolution as a Means to Create Enantioselective Enzymes for Use in Organic ChemistryApplied Molecular Evolution of Enzymes Involved in Synthesis and Repair of DNAEvolutionary Generation versus Rational Design of Restriction Endonucleases with Novel SpecificityEvolutionary Generation of Enzymes with Novel Substrate Specificities

Susanne Brakmann is head of the junior research group "Applied Molecular Evolution" at the University of Leipzig (Germany) and a Member of the Biotechnological-Biomedical Center of Leipzig. She studied Chemistry at the Technical University of Braunschweig where she received her diploma in 1988, moving afterwards to the University of Karlsruhe to work on her thesis under the supervision of Reinhold Tacke (Ph. D. 1991). She was postdoctoral fellow at the Max-Planck-Institute for Biophysical Chemistry in Göttingen where she worked with Manfred Eigen before she moved to Leipzig in 2001. She is interested in directed evolution as a tool for understanding and optimizing enzyme functions, focusing on nucleic acid polymerases and their biotechnological applications. *****Kai Johnsson is assistant professor for Bioorganic Chemistry at the Swiss Federal Institute for Technology (EPFL) where he heads the laboratory for protein engineering. Prior to joining EPFL, he was a junior group leader at Ruhr-University Bochum in Germany, after spending three and a half years in the laboratory of Prof. Peter G. Schultz (University of California, Berkeley) as a postdoctoral research fellow. Kai Johnsson studied chemistry and did his PhD with Prof. Steven Benner at ETH Zurich. Since the start of his PhD thesis, Kai Johnsson's research interests focus on biological chemistry and in particular enzyme mechanisms and protein chemistry. Prof. Johnsson is the inventor of the Covalys technology.