DNA Vaccines

DNA Vaccine Design.- DNA Vaccine Design.- Design of Plasmid DNA Constructs for Vaccines.- Vaccination With Messenger RNA.- A Stress Protein-Facilitated Antigen Expression System for Plasmid DNA Vaccines.- In Vitro Assay of Immunostimulatory Activities of Plasmid Vectors.- DNA Vaccine Delivery Systems.- Delivery of DNA Vaccines Using Electroporation.- Needle-Free Injection of DNA Vaccines: A Brief Overview and Methodology.- Needle-Free Delivery of Veterinary DNA Vaccines.- Surface-Modified Biodegradable Microspheres for DNA Vaccine Delivery.- A Dendrimer-Like DNA-Based Vector for DNA Delivery: A Viral and Nonviral Hybrid Approach.- Identification of Compartments Involved in Mammalian Subcellular Trafficking Pathways by Indirect Immunofluorescence.- DNA Vaccine Adjuvants and Activity Enhancement.- Adjuvant Properties of CpG Oligonucleotides in Primates.- Complexes of DNA Vaccines With Cationic, Antigenic Peptides Are Potent, Polyvalent CD8+ T-Cell-Stimulating Immunogens.- Prime-Boost Strategies in DNA Vaccines.- Modifying Professional Antigen-Presenting Cells to Enhance DNA Vaccine Potency.- Replicase-Based DNA Vaccines for Allergy Treatment.- DNA Vaccine Applications.- Immunological Responses of Neonates and Infants to DNA Vaccines.- DNA Vaccines for Allergy Treatment.- Protection From Autoimmunity by DNA Vaccination Against T-Cell Receptor.- The Use of Bone Marrow-Chimeric Mice in Elucidating Immune Mechanisms.- DNA Vaccine Production, Purification, and Quality.- A Simple Method for the Production of Plasmid DNA in Bioreactors.- Practical Methods for Supercoiled pDNA Production.- Production of Plasmid DNA in Industrial Quantities According to cGMP Guidelines.- Large-Scale, Nonchromatographic Purification of Plasmid DNA.- Assuring the Quality, Safety, and Efficacy of DNAVaccines.

From the reviews:"...does an excellent job of describing the current methodologies in their development, what clinical benefit can be expected, and what is known of their mode of action...has thoroughly covered the current state of the art." -Antiviral Research"The present book contains 41 chapters by experts in the field not only describing the state-of-the-art thinking on each topic, but, more importantly, providing a step-by-step recipe for constructing the cost effective plasmids and measuring host immune responses, both humoral and cellular, as well as all the stops in between including delivery, etc....an excellent roadmap as to how to proceed...This comprehensive collection of chapters is the first of its kind in the rapidly moving field to provide an excellent update of all of the recent advances and, more importantly, the actual technical approaches to developing vaccines. This is a book that should be in everyone's library who is interested in pursing studies employing DNA immunization and for graduate students interested in an up-to-date understanding of DNA vaccination." -FEBS Letters"Its 41 chapters provide a valuable introduction, a series of working protocols, and a set of heavily referenced reviews of state-of-the-art methods in the production, use, and analysis of DNA vaccines. The various chapters are especially fruitful in their presentation of detailed techniques for making buffers and complex biological reagents." -Modern Drug Discovery"Tecular Biology series, consists of 41 well written and referenced chapters dealing with certain aspects of DNA vaccination. The subjects addressed range from large scale plasmid isolation to DNA vaccination and autoimmunity, and illuminate the explosive growth this field has undergone in recent years. A substantial amount of the text concentrates on the practical aspects of processing DNA, quality control and immunological responses....In short, this book is a valuable addition to the library of all people

From the reviews:

"...does an excellent job of describing the current methodologies in their development, what clinical benefit can be expected, and what is known of their mode of action...has thoroughly covered the current state of the art." -Antiviral Research

"The present book contains 41 chapters by experts in the field not only describing the state-of-the-art thinking on each topic, but, more importantly, providing a step-by-step recipe for constructing the cost effective plasmids and measuring host immune responses, both humoral and cellular, as well as all the stops in between including delivery, etc....an excellent roadmap as to how to proceed...This comprehensive collection of chapters is the first of its kind in the rapidly moving field to provide an excellent update of all of the recent advances and, more importantly, the actual technical approaches to developing vaccines. This is a book that should be in everyone's library who is interested in pursing studies employing DNA immunization and for graduate students interested in an up-to-date understanding of DNA vaccination." -FEBS Letters

"Its 41 chapters provide a valuable introduction, a series of working protocols, and a set of heavily referenced reviews of state-of-the-art methods in the production, use, and analysis of DNA vaccines. The various chapters are especially fruitful in their presentation of detailed techniques for making buffers and complex biological reagents." -Modern Drug Discovery

"Tecular Biology series, consists of 41 well written and referenced chapters dealing with certain aspects of DNA vaccination. The subjects addressed range from large scale plasmid isolation to DNA vaccination and autoimmunity, and illuminate the explosive growth this field has undergone in recent years. A substantial amount of the text concentrates on the practical aspects of processing DNA, quality control and immunological responses....In short, this book is a valuable addition to the