Drug Design and Discovery

Virtual Screening for Lead Discovery.- Computer-Aided Drug Discovery and Development.- Using Active Site Mapping and Receptor-Based Pharmacophore Tools: Prelude to Docking and De Novo/Fragment-Based Ligand Design.- Methods for Evaluation of Structural and Biological Properties of Anti-Invasive Natural Products.- Solid-Phase Peptide Synthesis Using Microwave Irradiation.- Fluorescent and Lanthanide Labeling for Ligand Screens, Assays, and Imaging.- DNA-Directed Assembly Microarray for Protein and Small Molecule Inhibitor Screening.- Selection of Peptide Ligands for Human Placental Transcytosis Systems Using In vitro Phage Display.- Optimization of the Tetrazolium Dye (MTT) Colorimetric Assay for Cellular Growth and Viability.- Imaging NF- B Signaling in Mice for Screening Anti-Cancer Drugs.- Evaluation of Anticancer Agents Using Flow Cytometry Analysis of Cancer Stem Cells.- Chemical Screening with Zebrafish Embryos.- iTRAQTM Labeling Coupled with LC-MALDI Mass Spectrometry for Monitoring Temporal Response of Colorectal Cancer Cells to Butyrate Treatment.- Heterotypic Cell Adhesion Assay for the Study of Cell Adhesion Inhibition.- Evaluation of Antibacterial Activity of Proteins and Peptides Using a Specific Animal Model for Wound Healing.- In vitro Immunogenicity Risk Assessment of Therapeutic Proteins in Preclinical Setting.

From the reviews:"This volume is an easily accessible collection of protocols covering a broad range of different screening methodologies. ... The strong academic background of the authors makes this book highly recommendable to everyone involved in drug screening and has a particular appeal to an academic audience. ... It is highly recommended for all those who are performing drug screening or who aim to be doing so in the near future." (Robin Ketteler, British Journal of Clinical Pharmacology, June, 2011)