Genome Instability
Methods and Protocols
(Sprache: Englisch)
This volume presents forty-two methods and protocols to analyze diverse aspects of genome instability. Chapters detail mutagenesis and repair, methods to quantify and analyze the properties of DNA double-strand breaks, profile replication, replication...
Jetzt vorbestellen
versandkostenfrei
Buch (Gebunden)
Fr. 189.00
inkl. MwSt.
- Kreditkarte, Paypal, Rechnungskauf
- 30 Tage Widerrufsrecht
Produktdetails
Produktinformationen zu „Genome Instability “
Klappentext zu „Genome Instability “
This volume presents forty-two methods and protocols to analyze diverse aspects of genome instability. Chapters detail mutagenesis and repair, methods to quantify and analyze the properties of DNA double-strand breaks, profile replication, replication proteins strand-specifically, genome instability, fluorescence microscopic techniques, and genomic and proteomic approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Genome Instability: Methods and Protocols aims to provide a comprehensive resource for the discovery and analysis of the proteins and pathways that are critical for stable maintenance of the genome.
Inhaltsverzeichnis zu „Genome Instability “
1. The A-like Faker Assay for Measuring Yeast Chromosome III StabilityCarolina A. Novoa, J. Sidney Ang, and Peter C. Stirling
2. The Chromosome Transmission Fidelity Assay for Measuring Chromosome Loss in Yeast
Supipi Duffy and Philip Hieter
3. Measuring Mutation Rates using the Luria-Delbrück Fluctuation Assay
Gregory I. Lang
4. Molecular Genetic Characterization of Mutagenesis Using a Highly Sensitized Single-Stranded DNA Reporter System in Budding Yeast
Kin Chan
5. Analyzing Genome Rearrangements in Saccharomyces cerevisiae
Anjana Srivatsan, Christopher D. Putnam, and Richard D. Kolodner
6. High Resolution Mapping of Modified DNA Nucleobases using Excision Repair Enzymes
Monica Ransom, D. Suzi Bryan and Jay R. Hesselberth
7. Integrated Microarray-based Tools for Detection of Genomic DNA Damage and Repair Mechanisms Patrick van Eijk, Yumin Teng, Mark R. Bennet, Katie E. Evans, James R. Powell, Richard M. Webster, and Simon H. Reed
8. Study of UV-induced DNA Repair Factor Recruitment: Kinetics and Dynamics
Sertic S., Roma S., Plevani P., Lazzaro F. , and Muzi-Falconi M.
9. Inserting Site-specific DNA Lesions into Whole Genomes
Vincent Pagès and Robert P Fuchs
10. A qPCR-based Protocol to Quantify DSB Resection
Matteo Ferrari, Shyam Twayana, Federica Marini, and Achille Pellicioli
11. Alkaline Denaturing Southern Blot Analysis to Monitor Double-strand Break Processing
Chiara Vittoria Colombo, Luca Menin, and Michela Clerici
12. Single Molecule Analysis of Resection Tracks
Pablo Huertas and Andrés Cruz-García
13. Mapping DNA Breaks by Next-generation Sequencing Laura Baranello, Fedor Kouzine, Damian Wojtowicz, Kairong Cui, Keji Zhao,Teresa M Przytycka, Giovanni Capranico, and David Levens
14. Genome-wide Profiling of DNA Double-strand Breaks by the
... mehr
BLESS and BLISS Methods
Reza Mirzazadeh, Tomasz Kallas, Magda Bienko, and Nicola Crosetto
15. DNA Replication Profiling using Deep Sequencing
Xanita Saayman, Cristina Ramos-Pérez, and Grant W Brown
16. Quantitative Bromodeoxyuridine Immunoprecipitation Analyzed by High-Throughput Sequencing (qBrdU-seq or QBU)
Joanna E. Haye-Bertolozzi and Oscar M. Aparicio
17. Strand-specific Analysis of DNA Synthesis and Proteins Association with DNA Replication Forks in Budding Yeast
Chuanhe Yu, Haiyun Gan, and Zhiguo Zhang
18. Analysis of Replicative Polymerase Usage by Ribonucleotide Incorporation Andrea Keszthelyi, Izumi Miyabe, Katie Ptasinska, Yasukazu Daigaku, Karel Naiman, and Antony M. Carr
19. Dynamic Architecture of Eukaryotic DNA Replication Forks in vivo, Visualized by Electron Microscopy
Ralph Zellweger and Massimo Lopes
20. A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation
Nicolai B. Larsen, Ian D. Hickson, and Hocine W. Mankouri 21. Single Cell Gel Electrophoresis for the Detection of Genomic Ribonucleotides
Barbara Kind, Christine Wolf, Kerstin Engel, Alexander Rapp,M. Cristina Cardoso, and Min Ae Lee-Kirsch
22. Measuring the Levels of Ribonucleotides Embedded in Genomic DNA
Meroni A., Nava G.M., Sertic S., Plevani P., Muzi-Falconi M. and Lazzaro F.
23. Mapping Ribonucleotides Incorporated into DNA by Hydrolytic End-Sequencing
Clinton D. Orebaugh, Scott A. Lujan, Adam B. Burkholder, Anders R Clausen, and Thomas A. Kunkel
24. Detection of DNA-RNA Hybrids in vivo
María García-Rubio, Sonia I. Barroso, and Andrés Aguilera
25. Analysis of de novo Telomere Addition by Southern Blot
Diego Bonetti and Maria Pia Longhese
26. Assays to Study Repair of Inducible DNA Double Strand Breaks at Telomeres
Roxanne Oshidari and Karim Mekhail
27. Telomerase RNA Imaging in Budding Yeast and Human Cells by Fluorescent in situ Hybridization
David Guérit, Maxime Lalonde, and Pascal Chartrand
28. Methods to Study Repeat Fragility and Instability in Saccharomyces cerevisiae
Erica J. Polleys and Catherine H. Freudenreich
29. Quantitative Analysis of the Rates for Repeat-mediated Genome Instability in a Yeast Experimental System
Elina A. Radchenko, Ryan J. McGinty, Anna Y. Aksenova, Alexander J. Neil, and Sergei M. Mirkin1
30. Measuring Dynamic Behaviour of Trinucleotide Repeat Tracts in vivo in Saccharomyces cerevisiae
Gregory M. Williams and Jennifer A. Surtees
31. The Detection and Analysis of Chromosome Fragile Sites
Victoria A. Bjerregaard, Özgün Özer, Ian D. Hickson, and Ying Liu
32. Imaging of DNA Ultrafine Bridges in Budding Yeast
Oliver Quevedo and Michael Lisby
33. Detection of Ultra-Fine Anaphase Bridges
Anna H. Bizard, Christian F. Nielsen, and Ian D. Hickson
34. A Chromatin Fiber Analysis Pipeline to Model DNA Synthesis and Structures in Fission Yeast
Sarah A. Sabatinos and Marc D. Green
35. Long-term Imaging of DNA Damage and Cell Cycle Progression in Budding Yeast Using Spinning Disk Confocal Microscopy
Riccardo Montecchi and Etienne Schwob
36. The CellClamper: A Convenient Microuidic Device for Time-Lapse Imaging of Yeast
Gregor W. Schmidt, Olivier Frey, and Fabian Rudolf 37. Characterization of Structural and Configurational Properties of DNA by Atomic Force Microscopy
Alice Meroni, Federico Lazzaro, Marco Muzi-Falconi, and Alessandro Podestà
38. Genome-wide Quantitative Fitness Analysis (QFA) of Yeast Cultures
Eva-Maria Holstein, Conor Lawless, Peter Banks, and David Lydall
39. Rewiring the Budding Yeast Proteome using Synthetic Physical Interactions
Guðjón Ólafsson and Peter H. Thorpe
40. Reporter-based Synthetic Genetic Array Analysis: a Functional Genomics Approach for Investigating Transcript or Protein Abundance Using Fluorescent Proteins in Saccharomyces cerevisiae
Hendrikje Göttert, Mojca Mattiazzi Usaj, Adam P. Rosebrock, and Brenda J. Andrews
41. Statistical Analysis and Quality Assessment of ChIP-seq Data with DROMPA
Ryuichiro Nakato and Katsuhiko Shirahige
42. Quantitative Analysis of DNA Damage Signaling Responses to Chemical and Genetic Perturbations
Francisco M. Bastos de Oliveira, Dongsung Kim, Michael Lanz, and Marcus B. Smolka
Reza Mirzazadeh, Tomasz Kallas, Magda Bienko, and Nicola Crosetto
15. DNA Replication Profiling using Deep Sequencing
Xanita Saayman, Cristina Ramos-Pérez, and Grant W Brown
16. Quantitative Bromodeoxyuridine Immunoprecipitation Analyzed by High-Throughput Sequencing (qBrdU-seq or QBU)
Joanna E. Haye-Bertolozzi and Oscar M. Aparicio
17. Strand-specific Analysis of DNA Synthesis and Proteins Association with DNA Replication Forks in Budding Yeast
Chuanhe Yu, Haiyun Gan, and Zhiguo Zhang
18. Analysis of Replicative Polymerase Usage by Ribonucleotide Incorporation Andrea Keszthelyi, Izumi Miyabe, Katie Ptasinska, Yasukazu Daigaku, Karel Naiman, and Antony M. Carr
19. Dynamic Architecture of Eukaryotic DNA Replication Forks in vivo, Visualized by Electron Microscopy
Ralph Zellweger and Massimo Lopes
20. A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation
Nicolai B. Larsen, Ian D. Hickson, and Hocine W. Mankouri 21. Single Cell Gel Electrophoresis for the Detection of Genomic Ribonucleotides
Barbara Kind, Christine Wolf, Kerstin Engel, Alexander Rapp,M. Cristina Cardoso, and Min Ae Lee-Kirsch
22. Measuring the Levels of Ribonucleotides Embedded in Genomic DNA
Meroni A., Nava G.M., Sertic S., Plevani P., Muzi-Falconi M. and Lazzaro F.
23. Mapping Ribonucleotides Incorporated into DNA by Hydrolytic End-Sequencing
Clinton D. Orebaugh, Scott A. Lujan, Adam B. Burkholder, Anders R Clausen, and Thomas A. Kunkel
24. Detection of DNA-RNA Hybrids in vivo
María García-Rubio, Sonia I. Barroso, and Andrés Aguilera
25. Analysis of de novo Telomere Addition by Southern Blot
Diego Bonetti and Maria Pia Longhese
26. Assays to Study Repair of Inducible DNA Double Strand Breaks at Telomeres
Roxanne Oshidari and Karim Mekhail
27. Telomerase RNA Imaging in Budding Yeast and Human Cells by Fluorescent in situ Hybridization
David Guérit, Maxime Lalonde, and Pascal Chartrand
28. Methods to Study Repeat Fragility and Instability in Saccharomyces cerevisiae
Erica J. Polleys and Catherine H. Freudenreich
29. Quantitative Analysis of the Rates for Repeat-mediated Genome Instability in a Yeast Experimental System
Elina A. Radchenko, Ryan J. McGinty, Anna Y. Aksenova, Alexander J. Neil, and Sergei M. Mirkin1
30. Measuring Dynamic Behaviour of Trinucleotide Repeat Tracts in vivo in Saccharomyces cerevisiae
Gregory M. Williams and Jennifer A. Surtees
31. The Detection and Analysis of Chromosome Fragile Sites
Victoria A. Bjerregaard, Özgün Özer, Ian D. Hickson, and Ying Liu
32. Imaging of DNA Ultrafine Bridges in Budding Yeast
Oliver Quevedo and Michael Lisby
33. Detection of Ultra-Fine Anaphase Bridges
Anna H. Bizard, Christian F. Nielsen, and Ian D. Hickson
34. A Chromatin Fiber Analysis Pipeline to Model DNA Synthesis and Structures in Fission Yeast
Sarah A. Sabatinos and Marc D. Green
35. Long-term Imaging of DNA Damage and Cell Cycle Progression in Budding Yeast Using Spinning Disk Confocal Microscopy
Riccardo Montecchi and Etienne Schwob
36. The CellClamper: A Convenient Microuidic Device for Time-Lapse Imaging of Yeast
Gregor W. Schmidt, Olivier Frey, and Fabian Rudolf 37. Characterization of Structural and Configurational Properties of DNA by Atomic Force Microscopy
Alice Meroni, Federico Lazzaro, Marco Muzi-Falconi, and Alessandro Podestà
38. Genome-wide Quantitative Fitness Analysis (QFA) of Yeast Cultures
Eva-Maria Holstein, Conor Lawless, Peter Banks, and David Lydall
39. Rewiring the Budding Yeast Proteome using Synthetic Physical Interactions
Guðjón Ólafsson and Peter H. Thorpe
40. Reporter-based Synthetic Genetic Array Analysis: a Functional Genomics Approach for Investigating Transcript or Protein Abundance Using Fluorescent Proteins in Saccharomyces cerevisiae
Hendrikje Göttert, Mojca Mattiazzi Usaj, Adam P. Rosebrock, and Brenda J. Andrews
41. Statistical Analysis and Quality Assessment of ChIP-seq Data with DROMPA
Ryuichiro Nakato and Katsuhiko Shirahige
42. Quantitative Analysis of DNA Damage Signaling Responses to Chemical and Genetic Perturbations
Francisco M. Bastos de Oliveira, Dongsung Kim, Michael Lanz, and Marcus B. Smolka
... weniger
Bibliographische Angaben
- 2017, 1st ed. 2018, XVI, 663 Seiten, 100 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Marco Muzi Falconi, Grant W Brown
- Verlag: Springer, Berlin
- ISBN-10: 1493973053
- ISBN-13: 9781493973057
- Erscheinungsdatum: 20.10.2017
Sprache:
Englisch
Kommentar zu "Genome Instability"
0 Gebrauchte Artikel zu „Genome Instability“
Zustand | Preis | Porto | Zahlung | Verkäufer | Rating |
---|
Schreiben Sie einen Kommentar zu "Genome Instability".
Kommentar verfassen