Human Retrovirus Protocols

Methods for Virus Isolation and Detection.- Enhanced Culture Assay for Detection and Quantitation of Latently Infected, Resting CD4+ T-Cells Carrying Replication-Competent Virus in HIV-1-Infected Individuals.- Isolation, Propagation, and Titration of Human Immunodeficiency Virus Type 1 From Peripheral Blood of Infected Individuals.- Isolation of Human Immunodeficiency Virus Type 1 From Peripheral Blood Monocytes.- Isolation, Propagation, and HIV-1 Infection of Monocyte-Derived Macrophages and Recovery of Virus From Brain and Cerebrospinal Fluid.- Isolation and HIV-1 Infection of Primary Human Microglia From Fetal and Adult Tissue.- Isolation of Human Immunodeficiency Virus Type 1 From Semen and Vaginal Fluids.- Isolation and Quantification of HIV From Lymph Nodes.- Isolation of Human Immunodeficiency Virus Type 2 Biological Clones From Peripheral Blood Lymphocytes.- Isolation and Confirmation of Human T-Cell Leukemia Virus Type 2 From Peripheral Blood Mononuclear Cells.- Isolation of Foamy Viruses From Peripheral Blood Lymphocytes.- Alu-LTR Real-Time Nested PCR Assay for Quantifying Integrated HIV-1 DNA.- Quantification of HFV-Integrated DNA in Human Cells by Alu-LTR Real-Time PCR.- Detection of HIV-1 Provirus and RNA by In Situ Amplification.- Detection of HTLV-1 Gene on Cytologic Smear Slides.- Detection of HIV-2 by PCR.- Quantitation of HIV-1 Viral RNA in Blood Plasma and Genital Secretions.- Quantification of Proviral DNA Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR.- Plasma RNA Viral Load in HIV-1 Group O Infection by Real-Time PCR.- A New Combined HIV p24 Antigen and Anti-HIV-1/2/O Screening Assay.- A New Automated Fourth-Generation HIV Screening Assay With Sensitive Antigen Detection Module and High Specificity.- Detection of Antiviral Antibodies Using Enzyme-Linked Immunosorbent Assay.- Plaque-Reduction Assays for Human and Simian Immunodeficiency Virus Neutralization.- Detection of Drug-Resistant HIV-1 Strains.- Molecular

"More than 20 years after the discoveries of HTLV and HIV, there appears to be a need for a consolidated reference of many of the standard and not-so-standard protocols used to characterize the human retroviruses. This book, containing some 37 detailed protocols provided by multinational authors in two parts, does an admirable job. Part I, therefore, includes guidance on isolation of HTLV, HIV and Human Foamy Virus (HFV) from peripheral blood, lymph nodes, CNS tissue and genital secretions (semen, vaginal fluids). It also describes a variety of molecular methods (largely PCR-based) to assess viral levels, integration status and drug sensitivity, plus quantification by antigen detection. At some 313 pages, Part I forms the substantial portion of the book. Part II (168 pages) guides on assessing cell and receptor tropism, construction and utilization of molecular clones and also use of microarrays to look at host and viral gene expression. The book on the whole is well illustrated with clear photographs and diagrams to support the detailed methodology, and the references at the end of each protocol are comprehensive. "-SGM Quarterly