Identification of Transcribed Sequences

Introduction: Seven Blind Men and An Elephant.- From Genomic DNA to Transcribed Sequences.- Classical Approaches.- Identification of Genes and Construction of a Transcriptional Map in Xq28.- Use of cDNA Selection and Evolutionarily Conserved Sequences to Isolate Transcribed Sequences from Region Xp11.21.- Identification of cDNAs by Direct Hybridization Using Cosmid Probes.- Hybridization Based Approaches.- Hybridization Selection:.- Locus Specific Identification of Transcribed Sequences Using YACs and Whole Yeast Genomic DNA.- Towards a Transcriptional Map of Human Chromosome 21.- Isolation of Expressed Sequences from the Chromosome 17q21 BRCA1 Region by Magnetic Bead Capture.- Towards a Transcriptional Map of the q21-q22 Region of Chromosome 7.- Direct cDNA Selection Using Human and Mouse cDNAs:Application to Xq13.3 Chromosomal Region.- Variations on Hybridization Selection:.- A Sandwich-Hybridization Method for Specific and Efficient Selection of cDNA Clones from Genomic Regions.- Novel Strategy for Isolating Unknown Coding Sequences from Genomic DNA by Generating Genomic-cDNA Chimeras.- Identifying and Directly Purifying Transcribed Elements by Coincident Sequence Cloning.- Finding Candidate Genes by Preparative in situ Hybridization.- Direct cDNA Screening:.- Direct cDNA Screening of Genomic Reference Libraries - A Rapid Method for the Identification of Transcribed Sequences in Large Genomic Regions.- Identification of Expressed Sequences on Human Chromosome 9q32-34.- Exon Trapping.- An Exon Trapping System Providing Size Selection of Spliced Clones and Facilitating Direct Cloning.- Isolation of Gene Sequences from the BRCA1 Region of Chromosome 17q21 by Exon Amplification.- Isolation of Coding Sequence from Cosmids and YACs by Exon Amplification.- IntegratedTranscriptional Maps of Large DNA Regions: Towards a Transcriptional Map of Human Chromosome 21.- Computer Based Approaches.- Shallow Shotgun Sequencing as a Strategy for Finding Coding Exons.- From