Imaging Gene Expression

High-Throughput Fluorescence-Based Screen to Identify Factors Involved in Nuclear Receptor Recruitment to Response Elements.- Live-Cell Imaging Combined with Immunofluorescence, RNA, or DNA FISH to Study the Nuclear Dynamics and Expression of the X-Inactivation Center.- Single Molecule Resolution Fluorescent In Situ Hybridization (smFISH) in the Yeast S. cerevisiae.- Measuring Transcription Dynamics in Living Cells Using Fluctuation Analysis Nuclear Poly(A) RNA Movement Within and Among Speckle Nuclear Bodies and the Surrounding Nucleoplasm.- Nuclear Trafficking and Export of Single, Native mRNPs in Chironomus tentans Salivary Gland Cells.- Single mRNP Tracking in Living Mammalian Cells.- Imaging Nascent RNA Dynamics in Dictyostelium.- Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Single Molecule Tracking.- Single Particle Tracking for Studying the Dynamic Properties of Genomic Regions in Live Cells.- Microscopic Analysis of Chromatin Localization and Dynamics in C. elegans.- Measuring the Dynamics of Chromatin Proteins During Differentiation.- Electron Spectroscopic Tomography of Specific Chromatin Domains.- BAC Manipulations for Making BAC Transgene Arrays.- Spatio-Temporal Visualization of DNA Replication Dynamics.- The Dynamics of DNA Damage Repair and Transcription.- Fluorescence Microscopy-Based High-Throughput Screening for Factors Involved in Gene Silencing.- Actin as a Model for the Study of Nucleocytoplasmic Shuttling and Nuclear Dynamics.- Isochronal Visualization of Transcription and Proteasomal Proteolysis in Cell Culture or in the Model Organism, Caenorhabditis elegan.- Considering Discrete Protein Pools When Measuring the Dynamics of Nuclear Membrane Proteins.- Correlative Microscopy of Individual Cells: Sequential Application of Microscopic Systems with IncreasingResolution to Study the Nuclear Landscape.- Time-Lapse, Photo-Activation, and Photo-Bleaching Imaging of Nucleolar Assembly after Mitosis.- Nucleation of Nuclear Bodies.