In Situ Hybridization Protocols

Guidelines for the Optimization and Validation of In Situ Hybridization.- Overcoming Autofluorescence (AF) and Tissue Variation in Image Analysis of In Situ Hybridization.- Practical Application of Fluorescent In Situ Hybridization Techniques in Clinical Diagnostic Laboratories.- Fluorescent In Situ Hybridization using Oligonucleotide-Based Probes.- Visualizing Genome Reorganization using 3D DNA FISH.- MicroRNA In Situ Hybridization in Paraffin Embedded Cultured Cells.- Multiplexed Detection and Analysis of Low Abundant Abundance Long Non-Coding RNA using RNAscopeTM in Cultured Cells.- Multiplexed Quantitative In Situ Hybridization for Mammalian or Bacterial Cells in Suspension: qHCR Flow Cytometry (v3.0).- Multiplexed Quantitative In Situ Hybridization for Mammalian Cells on a Slide: qHCR and dHCR Imaging (v3.0).- Multiplexed Quantitative In Situ Hybridization with Subcellular or Single-Molecule Resolution within Whole-Mount VertebrateEmbryos: qHCR and dHCR imaging (v3.0).- Hybridization Chain Reaction for Quantitative and Multiplex Imaging of Gene Expression in Amphioxus Embryos and Adult Tissues.- RNAscopeTM Multiplex Detection in Zebrafish.- Duplex In Situ Hybridization of Virus Nucleic Acids in Plant Tissues using RNAscopeTM.- Automated ISH for Validated Histological Mapping of Lowly Expressed Genes.- Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping.- Automated Co-In Situ Hybridization and Immunofluorescence using Archival Tumor Tissue.- Automated 5-Color Multiplex Co-Detection of MicroRNA and Protein Expression in Fixed Tissue Specimens.- Mixed Multiplex Staining: Automated RNAscopeTM and OPALTM for Multiple Targets.- Simultaneous Visualization of RNA and Protein Expression in Tissue Using a Combined RNAscopeTM In Situ Hybridization and Immunofluorescence Protocol.- In