In Vitro Transcription and Translation Protocols

Technologies.- Energy Systems for ATP Regeneration in Cell-Free Protein Synthesis Reactions.- Continuous-Exchange Protein-Synthesizing Systems.- Cell-Free Production of Integral Membrane Proteins on a Preparative Scale.- SIMPLEX: Single-Molecule PCR-Linked In Vitro Expression.- Methods for High-Throughput Materialization of Genetic Information Based on Wheat Germ Cell-Free Expression System.- Exogenous Protein Expression in Xenopus Oocytes.- Applications.- In Vitro Translation to Study HIV Protease Activity.- The Protein Truncation Test in Mutation Detection and Molecular Diagnosis.- Creation of Novel Enantioselective Lipases by SIMPLEX.- In Vitro Transcription and Translation Coupled to Two-Dimensional Electrophoresis for Bacterial Proteome Analysis.- In Vitro Screen of Bioinformatically Selected Bacillus anthracis Vaccine Candidates by Coupled Transcription, Translation, and Immunoprecipitation Analysis.- Functional Expression of Type 1 Rat GABA Transporter in Microinjected Xenopus laevis Oocytes.- Production of Protein for Nuclear Magnetic Resonance Study Using the Wheat Germ Cell-Free System.