Jasmonate Signaling

Phenotyping Jasmonate Regulation of Senescence.- Characterizing Jasmonate Regulation of Male Fertility in Arabidopsis.- Phenotyping Jasmonate Regulation of Root Growth.- Bioassays for Assessing Jasmonate-Dependent Defenses Triggered by Pathogens, Herbivorous Insects, or Beneficial Rhizobacteria.- Elicitation of Jasmonate-Mediated Defense Responses by Mechanical Wounding and Insect Herbivory.- Pseudomonas syringae Infection Assays in Arabidopsis.- Jasmonate Signaling in the Field, Part I: Elicited Changes in Jasmonate Pools of Transgenic Nicotiana attenuata Populations.- Jasmonate Signaling in the Field, Part II: Insect-Guided Characterization of Genetic Variations in Jasmonate-Dependent Defenses of Transgenic and Natural Nicotiana attenuata Populations.- Profiling of Jasmonic Acid-Related Metabolites and Hormones in Wounded Leaves.- A Non-Targeted Approach for Extended Liquid Chromatography-Mass Spectrometry Profiling of Free and Esterified Jasmonates After Wounding.- Cell-Specific Detection of Jasmonates by Means of an Immunocytological Approach.- Jasmonic Acid-Amino Acid Conjugation Enzyme Assays.- Pull-Down Analysis of Interactions Among Jasmonic Acid Core Signaling Proteins.- Yeast Two-Hybrid Analysis of Jasmonate Signaling Proteins.- Modified Bimolecular Fluorescence Complementation Assay to Study the Inhibition of Transcription Complex Formation by JAZ Proteins.- Agroinfiltration of Nicotiana benthamiana Leaves for Co-Localization of Regulatory Proteins Involved in Jasmonate Signaling.- Electrophoretic Mobility Shift Assay for the Analysis of Interactions of Jasmonic Acid-Responsive Transcription Factors with DNA.- Transient Expression Assays in Tobacco Protoplasts.- Functional Analysis of Jasmonic Acid-Responsive Secondary Metabolite Transporters.- Expression Analysis of Jasmonate-Responsive Lectins in Plants.- Profiling the Jasmonic Acid Responses by Nuclear Magnetic Resonance-Based Metabolomics.- Metabolite Profiling of Plant Tissues by Liquid