Metastasis Research Protocols

In Vitro Invasion Assay Using MatrigelTM - a Reconstituted Basement Membrane Preparation.- Single Cell and Spheroid Collagen Type I Invasion Assay.- Rocking Adhesion Assay System to Study Adhesion and Transendothelial Migration of Cancer Cells.- Small Cell Lung Cancer (SCLC) Cell Adhesion on E- and P-Selectin under Physiological Flow Conditions.- Adhesion of Tumour Cells to Matrices and Endothelium.- Cell Aggregation Assays.- Chick Heart Invasion Assay.- Computer Simulation of the Metastatic Progression.- Theoretical Considerations In Using Animal Models of Metastasis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice.- Syngeneic Murine Metastasis Models: B16 Melanoma.- Imageable Clinically-relevant Mouse Models of Metastasis.- Imaging Metastatic Cell Trafficking at the Cellular Level in vivo with Fluorescent Proteins.- Ultrasound Techniques for the Detection of Tumours and Metastases in Small Animals.- The PFP/RAG2 Double Knock Out Mouse in Metastasis Research: Small Cell Lung Cancer and Prostate Cancer.- Ultrasound Guided Intracardial Injection and In-vivo Magnetic Resonance Imaging of Single Cells in Mice as a Paradigm for Haematogenous Metastases.- Magnetic Resonance Imaging of Metastases in Xenograft Mouse Models of Cancer.- Spontaneous and Experimental Metastasis Models: Nude Mice.- Identifying the Origin and Phenotype of Cells in Tumour Xenografts.