Metastasis Research Protocols

Basic Immunocytochemistry for Light Microscopy.-Lectin Histochemistry to Detect Altered Glycosylation in Cells and Tissues.-Histopathological Assessment of Metastasis.-Immunocytochemical Detection and Characterisation of Individual Micrometastatic Tumour Cells.-The Polymerase Chain Reaction (PCR).-SDS-PAGE and Western Blotting Techniques.-2-DE Based Proteomics for the Analysis of Metastasis Associated Proteins.-Assessment of Gelatinases (MMP-2 and MMP-9) by Gelatin Zymography.-Determination of Cell Specific Receptor Binding using a Combination of Immunohistochemistry and in vitro Autoradiography: Relevance to Therapeutic Receptor Targeting in Cancer.-Fluorescence in Situ Hybridization (FISH) for Cancer Related Studies.-Using a Quartz Crystal Microbalance Biosensor for the Study of Metastasis Markers on Intact Cells.-Cell Separations by Flow Cytometry.-Detection of Putative Cancer Stem Cells of the Side Population Phenotype in Human Tumor Cell Cultures.-Genes Involved in the Metastatic Cascade of Medullary Thyroid Tumours.-High-resolution Quantitative Methylation Analysis of microRNA Genes using Pyrosequencing(TM).-RNAi Technology to Block the Expression of Molecules Relevant to Metastasis: The Cell Adhesion Molecule CEACAM1 as an Instructive Example.-Galectin-3-Binding and Metastasis.-Lectin Array Based Strategies for Identifying Metastasis-associated Changes in Glycosylation.

From the book reviews:"This second edition ... describes cellular and molecular techniques used to study cancer metastasis. ... It is written for any researchers interested in cellular and molecular techniques relevant to cancer cells and tissue. ... This is a great addition to any laboratory studying cancer metastasis. ... I plan to have all my new lab members read through this book. Even those already working for years in the lab can learn new tips and techniques." (Jennifer E. Koblinski, Doody's Book Reviews, January, 2013)