Modern Biocatalysis

MediaILs/SCFs Biphasic Systems as Promising Media for BiocatalysisThe [bmim][PF6]/SC-CO2 System as a Reaction MediaEffect of Acyl Donor ConcentrationSTRATEGIES FOR THE BIOCATALYTIC LIPOPHILIZATION OF PHENOLIC ANTIOXIDANTSIntroductionMaterials and MethodsResults and DiscussionBIOCATALYSIS APPLIED TO THE SYNTHESIS OF NUCLEOSIDE ANALOGSIntroductionChemoenzymatic Modification of the SugarResolution and Anomeric SeparationBiotransformations that Modify the BaseTransglycosylation for the Synthesis of NucleosidesEFFICIENT FRUCTOOLIGOSACCHARIDE SYNTHESIS WITH A FRUCTOSYLTRANSFERASE FROM ASPERGILLUS ACULEATUSIntroductionPurification of Fructosyltransferase in Pectinex Ultra SP-LProperties of Fructosyltransferase from A. aculeatusImmobilization of Fructosyltransferase from A. aculeatusFructooligosaccharide Production Using Sugar Beet Syrup and MolassesHYDANTOIN RACEMASE: THE KEY ENZYME FOR THE PRODUCTION OF OPTICALLY PURE ALPHA-AMINO ACIDSIntroductionSearch for New Hydantoin Racemases and Molecular CharacterizationBiochemical Characterization of Hydantoin Racemase EnzymesSubstrate Enantioselectivity and Kinetic Analysis of Hydantoin RacemasesProposal for a Reaction Mechanism of Hydantoin Racemase EnzymesDesign of a Tailormade Recombinant Biocatalyst Including Hydantoin Racemase Enzymes for Optically Pure D-Amino Acid ProductionCHEMO-ENZYMATIC DERACEMIZATION METHODSIntroductionDeracemization Methods for Alpha- and Beta-Hydroxy AcidsDeracemization of Alpha-Hydroxy NitrilesDeracemization of Alpha-Amino AcidsUseful Enzymes for Deracemization MethodsNITRILASES FROM FILAMENTOUS FUNGIIntroductionDistribution and Evolutionary Relationship of Fungal NitrilasesStructural PropertiesCatalytic PropertiesNITRILASE- AND NITRILE HYDRATASE-CATALYZED ENANTIOSELECTIVE PREPARATION OF NON-PROTEINOGENIC AMINO ACIDSIntroductionNitrilase Hydratase/Amidase BiotransformationsNitrilase BiotransformationsNITRILASES IN THE ENANTIOSELECTIVE SYNTHESIS OF ALPHA-HYDROCYCARBOXYLIC ACIDSRoutes to Enantiomerically Pure Alpha-Hydroxycarboxylic AcidsNitrilase-Mediated Hydrolysis of CyanohydrinsA Bienzymatic Approach to Enantiopure 2-Hydrocycarboxylic AcidsStabilization of NLases as Cross-Linked Enzyme AggregatesHydrocyanation and Hydrolysis in a Bienzymatic CascadeNitrilases Acting as Nitrile HydratasesUF-MEMBRANE BIOREACTORS FOR KINETICS CHARACTERIZATION OF NITRILE HYDRATASE-AMIDASE-CATALYZED REACTIONS: A SHORT SURVEYIntroductionExperiment DesignTemperature Dependence of the Nitrile Hydratase-Amidase Cascade SystemCSMR InvestigationsSubstrate Concentration Effects on the Reaction Rate, Enzyme Stability, Substrate Conversion, and Reactor CapacityENZYMES CATALYZING C-C BOND FORMATION FOR THE SYNTHESIS OF MONOSACCHARIDE ANALOGSIntroductionRecent Syntheses Involving Transketolase and Fructose-1,6-bisphosphate AldolaseModification of Substrate Specificity of Yeast TransketolaseNOVEL STRATEGIES IN ALDOLASE-CATALYZED SYNTHESIS OF IMINOSUGARSIntroductionDHAP-Aldolase-Mediated Synthesis of Iminosugars from N-Cbz-Amino AldehydesD-Fructose-6-Phosphate Aldolase as Catalyst for Iminosugar SynthesisBIOCATALYTIC ASYMMETRIC OXIDATIONS WITH OXYGENIntroductionBiocatalytic Asymmetric Oxidations with OxidasesBiocatalytic Asymmetric Oxidations with PeroxidasesBiocatalytic Asymmetric Oxidations with DehydrogenasesBiocatalytic Asymmetric Oxidations with MonooxygenasesBiocatalytic Asymmetric Oxidations with DioxygenasesBiocatalytic Asymmetric Oxidations with Other EnzymsSECOND GENERATION BAEYER-VILLIGER BIOCATALYSTSIntroductionVBMO Enzyme PlatformEngineering of BVMOsBaeyer-Villiger Biooxidation in Synthetic ChemistryBVMOs in Stereoselective SulfoxidationsTowards a Technology Platform