Multiphoton Microscopy

Building a Two-Photon Microscope is Easy.- Using Multiphoton Imaging for Targeted Electrophysiological Recording and Live Cell Imaging of Fluorescently Labeled Neurons from Isolated Retinas.- Two-Photon Neurotransmitter Uncaging for the Study of Dendritic Integration.- Multiphoton Glutamate Uncaging to Study Structural and Functional Plasticity of Dendritic Spines.- Two-Photon Fluorescence Imaging of Visually Evoked Glutamate Release using iGluSnFR in the Mouse Visual System.- Imaging Neuronal Signal Transduction using Multiphoton FRET-FLIM.- Combining Multiphoton Excitation Microscopy with Fast Microiontophoresis to Investigate Neuronal Signaling.- Multiphoton Excitation Microscopy for Reconstruction and Analysis of Single Neuron Morphology.- Serial Multiphoton Tomography and Analysis of Volumetric Images of the Mouse Brain.- Studying a Light Sensor with Light: Multiphoton Imaging in the Retina.- Multiphoton Ca2+ Imaging of Astrocytes with Genetically Encoded Indicators Delivered by a Viral Approach.- Two-Photon Imaging of Dendritic Calcium Dynamics In Vivo.- Voltage Imaging with ANNINE Dyes and Two-Photon Microscopy.- High-Speed, Random-Access Multiphoton Microscopy for Monitoring Synaptic and Neuronal Activity in 3D in Behaving Animals.

"I recommend this exciting book, replete with full-color images, because of its comprehensive, tested protocols for the study of live neural tissue at the cellular and the subcellular levels." (Barry R. Masters, Optics & Photonics News, osa-opn.org, March 5, 2020)