Patch-Clamp Methods and Protocols

Pharmacology.- Pharmacological Analysis of Recombinant NR1a/2A and NR1a/2B NMDA Receptors Using the Whole-Cell Patch-Clamp Method.- Memantine as an Example of a Fast, Voltage-Dependent, Open Channel N-Methyl-d-Aspartate Receptor Blocker.- Methods for Evaluation of Positive Allosteric Modulators of Glutamate AMPA Receptors.- Automated Voltage-Clamp Technique.- Flip-the-Tip.- The Roboocyte.- Physiology.- Infrared-Guided Laser Stimulation as a Tool for Elucidating the Synaptic Site of Expression of Long-Term Synaptic Plasticity.- Single-Cell RT-PCR, a Technique to Decipher the Electrical, Anatomical, and Genetic Determinants of Neuronal Diversity.- Mechanosensitive Ion Channels Investigated Simultaneously by Scanning Probe Microscopy and Patch Clamp.- Synaptic Connectivity in Engineered Neuronal Networks.- Modeling of Action Potential Generation in NG108-15 Cells.- Whole-Cell Voltage Clamp on Skeletal Muscle Fibers With the Silicone-Clamp Technique.- Determination of Channel Properties at the Unitary Level in Adult Mammalian Isolated Cardiomyocytes.- Electrophysiological Properties of Embryonic Stem Cells During Differentiation Into Cardiomyocyte-Like Cell Types.- Hybrid Neuronal Network Studies Under Dynamic Clamp.- Cardiac Channelopathies Studied With the Dynamic Action Potential-Clamp Technique.- Biophysics.- Principles of Single-Channel Kinetic Analysis.- Use of Xenopus Oocytes to Measure Ionic Selectivity of Pore-Forming Peptides and Ion Channels.- Estimation of Quantal Parameters With Multiple-Probability Fluctuation Analysis.

From the reviews:"This book will be helpful to researchers already working in the field of electrophysiology, but also those coming to this field from pharmacological, physiological, and biophysical areas." - Doody's Book Review Service"'Patch-clamp: Methods and Protocols' introduces some common patch-clamp applications. It is intended to assist scientists in identifying problems, which could be best addressed by this technique. The book provides the step-by-step procedures to perform the experiments that answer those questions. ... It will be a useful companion for moderately advanced users wishing to extend their skills." (Maria Drigelova, General Physiology and Biophysics, Vol. 27, April, 2008)

From the reviews:

"This book will be helpful to researchers already working in the field of electrophysiology, but also those coming to this field from pharmacological, physiological, and biophysical areas." - Doody's Book Review Service

"'Patch-clamp: Methods and Protocols' introduces some common patch-clamp applications. It is intended to assist scientists in identifying problems, which could be best addressed by this technique. The book provides the step-by-step procedures to perform the experiments that answer those questions. ... It will be a useful companion for moderately advanced users wishing to extend their skills." (Maria Drigelova, General Physiology and Biophysics, Vol. 27, April, 2008)