Plant Proteostasis

Approaches to Determine Protein Ubiquitination ResidueTypes.- Immunoprecipitation of Cullin Ring Ligases(CRLs) in Arabidopsis Thaliana Seedlings.- Radioligand Binding Assays for Determining Dissociation Constants ofPhytohormone Receptors.- Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes.- Fluorescent Reporters for Ubiquitin-Dependent Proteolysis in Plants.- Generation of Artificial N-end Rule Substrate Proteins In Vivo and In Vitro.- Peptide Arrays for Binding Studies of E3 Ubiquitin Ligases.- SUMO ChainFormation by Plants Enzymes.- Kinetic Analysis of Plant SUMO Conjugation Machinery.- Expression, Purification, and EnzymaticAnalysis Of Plant SUMO Proteases.- Quantitative Analysis ofSubcellular Distribution of the SUMO Conjugation System by Confocal MicroscopyImaging.- Biochemical Analysis of Autophagy in Algae and Plants by Monitoringthe Electrophoretic Mobility of ATG8.- Detection of Autophagy inPlants by Fluorescence Microscopy.- Protocols for Studying Protein Stability in a Arabidopsis Protoplast Transient Expression System.- Detection and Quantification of Protein Aggregates in Plants.- Determination of ProteinCarbonylation and Proteasome Activity in Seeds.- Isobaric Tag for Relativeand Absolute Quantitation (iTRAQ)-based Protein Profiling in Plants.-Use of a Phosphatidylinositol phosphate AffinityChromatography (PIP - chromatography) for the Isolation of Proteins Involved inProtein Quality Control and Proteostasis Mechanisms in Plants.- In vivo Radiolabeling of Arabidopsis Leaves and Separation of ThylakoidMembrane Complexes by Blue Native PAGE.- Normalized Quantitative Western BlottingBased on Standardized Fluorescent Labeling.- Sequence Search andComparative Genomic Analysis of SUMO-Activating Enzymes using CoGe.- Studying Evolutionary Dynamicsof Gene Families Encoding SUMO-Activating Enzymes with SeaView and ProtTest.- Bioinformatics