Plant Vacuolar Trafficking

Green-fluorescent Seed, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds.- Detection of CRISPR/Cas9 Induced Genomic Fragment Deletions in Barley and Generation of Homozygous Edited Lines via Embryogenic Pollen Culture.- Vacuolar Sorting Determinants - Isolation and Study.- Vacuole-targeted Proteins: Ins and Outs of Subcellular Localization Studies.- Plant Cell Vacuoles: Staining and Fluorescent Probes.- Vacuolar Targeting and Characterization of Recombinant Antibodies.- Isolation of Vacuoles from the Leaves of the Medicinal Plant Catharanthus roseus.- Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells.- Live cell Imaging and Confocal Microscopy.- Imaging Vacuolar Anthocyanins with Fluorescence Lifetime Microscopy (FLIM).- The Use of Drugs in the Study of Vacuole Morphology and Trafficking to the Vacuole in Arabidopsis thaliana.- Use of Brefeldin A and Wortmannin to Dissect Post-Golgi OrganellesRelated to Vacuolar Transport in Arabidopsis thaliana.- Use Endosidin2 to Study Plant Exocytosis and Vacuolar Trafficking.- Visualization of RMRs (Receptor Membrane RING-H2) Dimerization in Nicotiana Benthamiana Leaves using a Bimolecular Fluorescence Complementation (BiFC) Assay.- Vacuole Dynamics in Rice Cells Invaded by the Blast Fungus Magnaporthe Oryzae.- Characterization Of Plant Glycoproteins: Analysis of Plant Glycopeptide Mass Spectrometry Data with plantGlycoMS, a Package in the R Statistical Computing Environment.