Protein Sequencing Protocols

Strategies for Handling Polypeptides on a Micro-Scale.- SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing.- Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization.- Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization.- Enzymatic Cleavage of Proteins.- Chemical Cleavage of Polypeptides.- Enzymatic Preparation and Isolation of Glycopeptides.- Analytical and Micropreparative Capillary Electrophoresis of Peptides.- High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues.- Hydrolysis of Samples for Amino Acid Analysis.- Amino Acid Analysis.- Post Column Amino Acid Analysis.- Amino Acid Analysis Using Pre-Column Derivatization with 6-AminoquinolylN-Hydroxysuccinimidyl Carbamate.- Amino Acid Analysis in Protein Hydrolysates Using Anion Exchange Chromatography and IPAD Detection.- D-Amino Acid Analysis.- Validation of Amino Acid Analysis Methods.- Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins.- Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry.- Nanoelectrospray Tandem Mass Spectrometry and Sequence Similarity Searching for Identification of Proteins from Organisms with Unknown Genomes.- Direct Identification of Proteins in Ultracomplex Mixtures.- Identification of PTH-Amino Acids by HPLC.- Protein Sequencer Maintenance and Troubleshooting.- N-Terminal Protein Sequencing for Special Applications.- Identification of Phosphorylation Sites by Edman Degradation.- Validation of Protein Sequencing in a Regulated Laboratory.- Automated C-Terminal Protein Sequence Analysis Using the Alkylated-Thiohydantoin Method.- Chemical Modifications of Proteins as anAid to Sequence Analysis.- Deblocking of N-Terminally Modified Proteins.- Deblocking of Proteins Containing N-Terminal PyroglutamicAcid.- Identification of Sites of Glycosylation.- Analysis of Sites

"...this book would certainly benefit readers who are new to the field as well as those more experienced as it serves as a convenient collection of tried and trusted methods...the book gives valuable background and insight to the various techniques associated with protein sequencing, which are often used by those providing services from within a core facility to the wider population of life science researchers." -Proteomics

"A positive feature of this book is that the fact that minute details, which are essential for successful implementation of an analytical method are provided. This book can serve as a useful reference for laboratories which mainly utilize chemical methods for protein analysis." -Pharmaceutical Research

From Reviews of the First Edition...

"...full of valuable information..." -Biochemical Education
"...provides very useful resources for those embarking on protein identification or characterization projects..." -FEBS Letters
"...an extremely useful compendium of protein analysis protocols..." -Nahrung-Food
"...well organized and accessible..." -Journal of the American Chemical Society

"The overall result is a concise and highly informative laboratory manual aimed at both operators and users of protein analysis facilities providing an understanding of what techniques involve, how samples should be prepared and in what form and amounts they should be submitted to achieve the most successful data. This publication has certainly proved to be a valuable asset to my laboratory and I would recommend it not only to those in protein sequencing and mass spectrometry laboratories, but also anyone with protein chemistry applications and interests."-Molecular Biotechnology