Quantitative Real-Time PCR

Twenty Years of qPCR: A Mature Technology?.- Minimum Information Necessary for Quantitative Real-Time PCR Experiments.- Selection of Reliable Reference Genes for RT-qPCR Analysis.- Introduction to Digital PCR.- mRNA and micro RNA Purity and Integrity: The Key to Success in Expression Profiling.- Mediator Probe PCR: Detection of Real-Time PCR by Label Free Probes and a Universal Fluorogenic Reporter.- Absolute Quantification of Viral DNA: The Quest for Perfection.- A Multiplex Real-Time PCR-Platform Integrated into Automated Extraction Method for the Rapid Detection and Measurement of Oncogenic HPV Type-Specific Viral DNA Load from Cervical Samples.- Real-Time PCR Detection of Mycoplasma pneumoniae in the Diagnosis of Community-Acquired Pneumonia.- A Sensible Technique to Detect Mollicutes Impurities in Human Cells Cultured in GMP Condition.- Real-Time Quantification Assay to Monitor BCR-ABL1 Transcripts in Chronic Myeloid Leukemia.- A Reliable Assay for Rapidly Defining Transplacental Metastasis Using Quantitative PCR.- Circulating Cell-Free DNA in Cancer.- Gene Expression Analysis by qPCR in Clinical Kidney Transplantation.- Post-Transcriptional Regulatory Networks: From Expression Profiling to Integrative Analysis of mRNA and Micro RNA Data.- Clinical Applications Using Digital PCR.- Developing Non-Invasive Diagnosis for Single Gene Disorders: The Role of Digital PCR.

From the book reviews:"The book is a valuable collection of qPCR-related details that cover a wide range of PCR uses. Aimed mainly at researchers (wet lab biologists) with a solid PCR background, the book provides enough details for the novice too, who may find the numerous examples as well as the end notes in each chapter very useful." (Irina Ioana Mohorianu, zbMATH, Vol. 1295, 2014)