Sample Preparation Techniques for Soil, Plant, and Animal Samples

Contents

Foreword

Ljubisav Rakic

1. Physical and Chemical Properties of Nucleic Acids

Daphne Real

2. Sampling for Biostatistics

Angela Conley and Jason Pfefferkorn

3. Sampling of Microbiological Samples

Christina Stam, Alberto Behar, and Moogega Cooper

4. Techniques for Nucleic Acid Purifications from Plant, Animal, and Microbial Samples

Jordan Ruggieri, Ryan Kemp, Stanislav Forman, and Marc E. Van Eden

5. Magnetic beads based nucleic acids purification for molecular biology applications

Nives Kovacevic

6. Systems for Mechanical Sample Lysis and Homogenization for Molecular Biology Applications

James A. Atwood III

7. Bead beating offers high performance homogenization for molecular biology downstream processing of tough and difficult samples

Vanessa M. Yu

8. High Performance Bead Beating Based Lysing, Homogenization and Grinding for DNA, RNA and proteins extraction with FastPrep® Systems

Miodrag Micic, Jeffrey D. Whyte, and Véronique Karsten

9. Portable Systems for Sample Lysis and Homogenization

Horacio Kido

10. DNA and RNA extractions from mammalian samples

Shuko Harada

11. Sample preparation for multiplex PCR assays for food and agriculture applications

Rachel Binet and Aparna Tatavarthy

12. Nucleic acid sample preparation from stem cells

Mirjana Pavlovic

13. Nucleic Acids Sample Preparation from Teeth/Dental Remains

Denice Higgins and Jeremy J. Austin

14. Nucleic Acids Sample Preparation for Food Traceability

Leonor Pereira, Sónia Gomes, and Paula Martins-Lopes

15. Nucleic Acids Sample Preparation for Quantitative and Qualitative GMO analysis

Maher Chaouachi

16. Nucleic Acids Samples Preparation from Dairy Products and Milk

Julie Ann Kase and Tina Lusk

17. Extraction of DNA from Plant Tissue: Review and Protocols

Farshad Tamari and Craig S. Hinkley

18. DNA Extraction from Seeds

Célio Dias Santos Júnior, Natália Melquie Monteiro

Miodrag "Mickey" Micic , Ph.D., Sc.D., M.T.M., C.S.W.P. is the Department Chair and Professor at the Department of Engineering Design Technology and Department of New Products Development at Cerritos College. He is also a Project Scientist at the BioMEMS Lab, Department of Mechanical and Aerospace Engineering, University of California, Irvine. He is a Visiting Professor at the Faculty of Applied Ecology-Futura at Singidunum University, Belgrade, Serbia. Dr. Micic is a consultant to several life sciences, medical devices, and biotech companies. Prior to his current position at Cerritos College, Dr. Micic was the Vice President of Research and Development at MP Biomedicals LLC (Formerly ICN Biomedicals Inc), Irvine, CA, USA. Earlier in his career, he was a Senior Application Scientist at Veeco Metrology, Santa Barbara, CA, USA. Dr. Micic did his postdoctoral work as a Postdoctoral Research Fellow at Pacifi c Northwest National Laboratories, Richland, WA, USA. He is the author of 67 publications in leading scientific journals, with over 1,400 citations. Dr. Micic holds a Ph.D. and M.S. degrees in Chemistry from the University of Miami, Coral Gables, FL, USA. He holds an M.T.M. degree from Washington State University, WA, USA. Further, Dr. Micic holds Sc.D. and M.Sc. degrees in Physical Chemistry; a B.Sc./B.Ed. in Physics and Fundamentals of Engineering from the University of Belgrade, Belgrade, Serbia; and a B.Eng. degree in Mechanical/Naval Architecture Engineering from the Polytechnic Academy of New Belgrade, Belgrade, Serbia. He is also an FAA licensed instrument-rated commercial pilot and a PADI master scuba diver.

"This monograph summarises and presents in an organised single text scattered relevant background information concerning sample preparation for a variety of molecular biology analyses, together with a number of protocols for successful treatment of difficult matrices. This book can be considered a useful practical guide for all those involved in sample preparation within the molecular biology practice, but it is also suitable for newcomers trying to obtain a general, but complete overview of the subject." (Lourde Ramos, Chromatographia, Vol. 79, 2016)