siRNA Design

What Parameters to Consider and Which Software Tools to Use for Target Selection and Molecular Design of Small Interfering RNAs.- Methods for Selecting Effective siRNA Target Sequences Using a Variety of Statistical and Analytical Techniques.- Designing Functional siRNA with Reduced Off-Target Effects.- Design and Screening of siRNAs Against Highly Structured RNA Targets.- Engineering Small Interfering RNAs by Strategic Chemical Modification.- The Design, Selection and Evaluation of Highly Specific and Functional siRNA Incorporating Unlocked Nucleobase Analogs.- The Design, Preparation, and Evaluation of Asymmetric Small Interfering RNA for Specific Gene Silencing in Mammalian Cells.- Design of Nuclease-Resistant Fork-Like Small Interfering RNA (fsiRNA).- Designing Dual-Targeting siRNA Duplexes Having Two Active Strands that Combine siRNA and MicroRNA-Like Targeting.- Strategies for Designing and Validating Immunostimulatory siRNAs.- Designing Efficient and Specific Endoribonuclease Prepared siRNAs (esiRNAs).- Short Hairpin RNA-Mediated Gene Silencing.- Design of Lentivirally Expressed siRNAs.- Bi-Functional Short Hairpin RNA (bi-shRNA): Design and Pathway to Clinical Application.- Design and Chemical Modification of Synthetic Short shRNAs as Potent RNAi Triggers.- Production and Application of Long dsRNA in Mammalian Cells.- Design of RNAi Reagents for Invertebrate Model Organisms and Human Disease Vectors.- Construction of shRNA Expression Plasmids for Silkworm Cell Lines Using Single-Stranded DNA and Bst DNA Polymerase.- Designing Effective amiRNA and Multimeric amiRNA Against Plant Viruses.- Downregulation of Plant Genes with MiRNA Induced Gene Silencing.

From the reviews:"This book edited by D.J. Taxman is a valuable collection of 20 chapters covering aspects from values for parameters used in tools for designing siRNAs to screening methods for evaluating the efficiency of the designed siRNA. ... Through style and scientific information the book is aimed at researchers in the RNAi field, but its accessible delivery of contents makes it a good starting point for younger researchers in this topic." (Irina Ioana Mohorianu, zbMATH, Vol. 1276, 2014)