SUMO

Concepts and Methodologies to Study Protein SUMOylation: An Overview.- The Regulation of Chromatin by Dynamic SUMO Modifications.- Reconstitution of the Recombinant RanBP1 SUMO E3 Ligase Complex.- Production and Purification of Recombinant SUMOylated Proteins Using Engineered Bacteria.- A Fluorescent In Vitro Assay to Investigate Paralog Specific SUMO Conjugation.- Identification and Characterization of SUMO-SIM Interactions.- Real-Time Surface Plasmon Resonance (SPR) for the Analysis of Interactions between SUMO-Traps and Mono or PolySUMO Moieties.- Using Biotinylated SUMO-Traps to Analyze SUMOylated Proteins.- In Vitro Characterization of Chain Depolymerization Activities of SUMO-Specific Proteases.- Detection of Protein SUMOylation In Situ by Proximity Ligation Assays.- In Situ SUMOylation and DeSUMOylation Assays: Fluorescent Methods to Visualize SUMOylation and DeSUMOylation in Permeabilized Cells.- Analysis of SUMOylated Proteins in Cells and In Vivo Using BioSUMO Strategy.- Label-Free Identification and Quantification of SUMO Target Proteins.- The Use of Multimeric Protein Scaffolds for Identifying Multi-SUMO Binding Proteins.- Isolation of In Vivo SUMOylated Chromatin-Bound Proteins.- Identification of Substrates of Protein-Group SUMOylation.- Tools to Study SUMO Conjugation in Caenorhabditis elegans.- Purification of SUMO Conjugates from Arabidopsis for Mass Spectrometry Analysis.- Detection of SUMOylation in Plasmodium falciparum.- Systematic Localization and Identification of SUMOylation Substrates in Knock-In Mice Expressing Affinity-Tagged SUMO1.