T-Cell Trafficking

Introductory Review.- How T Cells Find Their Way Around.- Migration of T Cells In Vitro.- Live Imaging of Leukocyte-Endothelium Interactions.- Leucocyte Adhesion Under Haemodynamic Flow Conditions.- Influence of Stromal Cells on Lymphocyte Adhesion and Migration on Endothelial Cells.- Discriminating Between the Paracellular and Transcellular Routes of Diapedesis.- Monitoring RhoGTPase Activity in Lymphocytes.- Visualisation of Signalling in Immune Cells.- Methods for Quantitation of Leukocyte Chemotaxis and Fugetaxis.- Analysis of CXCR3 and Atypical Variant Expression and Signalling in Human T Lymphocytes.- Transfection of Indoleamine 2,3 Dioxygenase in Primary Endothelial Cells.- Migration of T Cells In Vivo.- Visualisation of Lymphoid Organ Development.- Single-Cell Analysis of Cytotoxic T Cell Function by Intravital Multiphoton Microscopy.- Imaging Interactions Between the Immune and Cardiovascular Systems In Vivo by Multiphoton Microscopy.- Applying an Adaptive Watershed to the Tissue Cell Quantification During T-Cell Migration and Embryonic Development.- Monitoring T-Cell Migration in Human Diseases.- Identifying Homing Interactions in T-Cell Traffic in Human Disease.- Tracking Antigen-Experienced Effector T Cells In Vitro and In Vivo.- Preclinical Testing of Strategies for Therapeutic Targeting of Human T-Cell Trafficking In Vivo.

From the reviews:"This book, as the title describes, is primarily a collection of methods to investigate T-cell trafficking. ... this book details a comprehensive range of techniques for monitoring T-cell trafficking, along with the background behind the techniques, as well as their potential uses. This book would appeal to anyone interested in the field of T-cell trafficking and would be invaluable to PhD students and postdoctoral scientist setting up these techniques." (Judith Ramage, Immunology News, August, 2011)