T. cruzi Infection
Methods and Protocols
(Sprache: Englisch)
This volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T. cruzi and its host. Chapters detail a series of methods and protocols, ranging from rather classical...
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This volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T. cruzi and its host. Chapters detail a series of methods and protocols, ranging from rather classical biochemical/genetic approaches to modern, powerful and high-throughput '-omics' technologies, to tackle this issue, as well as novel techniques to improve treatment and clinical evaluation of Chagasic patients. Genome-wide mining strategies aimed at identifying potential parasite antigens and vaccine candidates and an overview on the challenges and milestones encountered during T. cruzi genome assembly are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, T. cruzi Infection: Methods and Protocols aims to provide students, researchers, and clinicians who are interested in the study of Chagas disease an indispensable source of information.
Inhaltsverzeichnis zu „T. cruzi Infection “
Preface...Table of Contents...Contributing Authors... 1. Trypanosoma cruzi Genome Assemblies: Challenges and Milestones of Assembling a Highly Repetitive and Complex GenomeJoão Luís Reis-Cunha and Daniella C. Bartholomeu 2. Mining Trypanosoma cruzi Genome Sequences for Antigen Discovery and Vaccine DevelopmentChristian Teh-Poot and Eric Dumonteil 3. Transcriptome Studies in Trypanosoma cruzi using RNAseqGonzalo Greif, Luisa Berná, Florencia Díaz-Viraqué, and Carlos Robello 4. An Improved Approach to Trypanosoma cruzi Molecular Genotyping by Next-Generation Sequencing of the Mini-Exon GeneAlicia Majeau, Claudia Herrera, and Eric Dumonteil 5. Genome Editing by CRISPR/Cas9 in Trypanosoma cruziNoelia Lander, Miguel A. Chiurillo, and Roberto Docampo 6. Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes Carlos André Ornelas Ricart, Rayner Myr Lauterjung Queiroz, Agenor de Castro Moreira dos Santos Júnior, and Sébastien Charneau 7. Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruziIzadora Volpato Rossi, Bruno Gavinho, and Marcel Ivan Ramirez 8. Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae María José Ferrer, Diana Patricia Wehrendt, Mariana Bonilla, Marcelo Alberto Comini, María Teresa Tellez-Iñón, and Mariana Potenza 9. Homologous Expression of Glycosylphosphatidyl Inositol-Anchored Glycoproteins in Trypanosoma cruzi Virginia Balouz, Andrea C. Mesias, Camila Centeno Camean, Ivana Ducrey, Maite Mabel Lobo, Ignacio M. Durante, Gaspar E. Cánepa, Carlos A. Buscaglia, and María de los Milagros Cámara 10. Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi trans-SialidaseGiannina Carlevaro, Andrés B. Lantos, Gaspar E. Cánepa, María de los Milagros Cámara, Martín Somoza, Carlos A. Buscaglia, Oscar Campetella, and Juan Mucci 11. Exploiting Genetically Modified Dual-Reporter Strains to Monitor Experimental Trypanosoma cruzi Infections and
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Host: Parasite Interactions Martin C. Taylor, Amanda F. Francisco, Shiromani Jayawardhana, Gurdip Singh Mann, Alexander Ward, Francisco Olmo, Michael D. Lewis, and John M. Kelly 12. Transmigration of Trypanosoma cruzi trypomastigotes through 3D Spheroids Mimicking Host TissuesMatías Exequiel Rodríguez, Mariana Rizzi, Lucas Caeiro, Yamil Masip, Daniel O. Sánchez, and Valeria Tekiel 13. In Situ Detection of Dormant Trypanosoma cruzi Amastigotes using Bioluminescent-Fluorescent ReportersFernando Sánchez-Valdéz and Angel Padilla 14. Multi-Parametric Evaluation of Trypanosoma cruzi Infection Outcome in Animal ModelsJulien Santi-Rocca, Núria Gironès, and Manuel Fresno 15. MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected MiceLudmila Rodrigues Pinto Ferreira 16. Genotyping of Trypanosoma cruzi from Clinical Samples by Multilocus Conventional PCR Otacilio C. Moreira and Juan Carlos Ramirez 17. Assessing Parasite Load in Chagas Disease Patients by Quantitative Multiplex Real-Time PCRJuan Carlos Ramírez and Otacilio da Cruz Moreira 18. Diagnostic Applicability of Neutralizing Antibodies to Trypanosoma cruzi trans-sialidase Leguizamón María Susana 19. Analytics of Functional Autoantibodies in Patients with Chagas' DiseaseGerd Wallukat, Katrin Wenzel, and Ingolf Schimke 20. Apolipoprotein-A1 and Fibronectin Fragments as Markers of Cure for the Chagas DiseaseElizabeth Ruiz-Lancheros, Makan Golizeh, and Momar Ndao 21. Host Derived Molecules as Novel Chagas Disease Biomarkers: Hypercoagulability Markers in PlasmaJulio Alonso-Padilla, Dolors Tassies, Nuria Cortes-Serra, Joaquim Gascon, Joan-Carles Reverter, and María-Jesús Pinazo 22. Purification of GlycosylphosphatidyLinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of ¿-Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas DiseaseUriel Ortega-Rodriguez, Susana Portillo, Roger A. Ashmus, Jerry A. Duran, Nathaniel S. Schocker, Eva Iniguez , Alba L. Montoya, Brenda G. Zepeda, Janet J. Olivas, Nasim H. Karimi, Julio Alonso-Padilla, Luis Izquierdo, Maria-Jesús Pinazo, Belkisyolé Alarcón de Noya , Oscar Noya, Rosa A. Maldonado, Faustino Torrico, Joaquim Gascón , Katja Michael, and Igor C. Almeida 23. Cytometric Bead Array (CBA) for Measurement Cytokines Levels in Chagas Disease PatientsNayara I. Medeiros and Juliana A. S. Gomes 24. Generation of T. cruzi-specific Primary CD4+ T Cell Lines from Peripheral Blood Mononuclear Cells Isolated from Chagas Disease Patients Gonzalo R. Acevedo, Paula B. Alcaráz, Clemencia Pinilla, and Karina A. Gómez 25. Trypanosma cruzi Specific T-Cell Responses to Monitor Treatment Efficacy in Chronic Chagas DiseaseMaría Cecilia Albareda, María Ailén Natale, Gonzalo Leandro Cesar, Melisa Daiana Castro Eiro, María Gabriela Alvarez, and Susana Adriana Laucella 26. CD8+ T Cell Response to Trypanosoma cruzi Antigens during Chronic Chagas DiseasePaola Lasso, Jose Mateus, John Mario González, Adriana Cuéllar, and Concepción Puerta 27. Fluorescence Proteomic Technology to Analyze Peripheral Blood Mononuclear Cells in Chronic Chagas Disease John E. Wiktorowicz, M. Paola Zago, and Nisha J. Garg 28. Isolation and Phenotypic Characterization of Inflammatory Cells from Clinical Samples: Purification of Macrophages from Trypanosoma cruzi-Infected HeartsNatalia Eberhardt, Liliana María Sanmarco, and Maria Pilar Aoki
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Bibliographische Angaben
- 2019, 1st ed. 2019, 398 Seiten, 47 farbige Abbildungen, Masse: 18,3 x 26 cm, Gebunden, Englisch
- Herausgegeben: Karina Andrea Gómez, Carlos Andrés Buscaglia
- Verlag: Springer, Berlin
- ISBN-10: 1493991477
- ISBN-13: 9781493991471
- Erscheinungsdatum: 14.03.2019
Sprache:
Englisch
Pressezitat
"The book is written for students, active researchers, and clinicians. ... This book comprehensively covers the experimental methods for the study of the biology of T. cruzi and its complex interactions with the human host. The unique feature of the book is that the variety of methods and protocols it covers. There are very few books that provide the depth of coverage this book does." (Omer Iqbal, Doody's Book Reviews, August 2019)
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